Open AccessHPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of Artemisia annua
Author(s) -
Jovana Stanković Jeremić,
Dejan Gođevac,
Stefan Ivanović,
Katarina Simić,
Антоанета Трендафилова,
Milica Aćimović,
Slobodan Milosavljević
Publication year - 2022
Publication title -
journal of the serbian chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.227
H-Index - 45
eISSN - 1820-7421
pISSN - 0352-5139
DOI - 10.2298/jsc210507007s
Subject(s) - metabolomics , artemisia annua , chromatography , derivatization , metabolite , chemistry , metabolome , thin layer chromatography , mass spectrometry , principal component analysis , artemisinin , biochemistry , biology , computer science , artificial intelligence , malaria , immunology , plasmodium falciparum
The application of High Performance Thin Layer Chromatography (HPTLC)-based non-targeted metabolomics as a holistic approach to compare fingerprints of metabolite changes during Artemisia annua development is described. Images of HPTLC chromatograms obtained after derivatization with anisaldehyde-sulphuric acid reagent were used as a dataset for multivariate analysis. Principal component analysis and Orthogonal Partial Least Squares Discriminant Analysis confirmed the discrimination of samples belonging to vegetative phase, flowering stage, and seed formation stage of the plant development. The obtained results showed that the HPTLC-based metabolomics approach can be a very reliable technique for the investigation of metabolic changes during plant development, complementary to GC-MS and NMR-based metabolomics.