Open AccessDNA protective activity of triterpenoids isolated from medicinal mushroom Fomitopsis betulina
Author(s) -
V Ivana Sofrenic,
D Boban Andjelkovic,
Ljubodrag Vujisić,
Miroslav Novaković,
Željka Knežević,
Bratislav Stanković,
Milosav Milosavljević,
Vele Tešević
Publication year - 2021
Publication title -
journal of the serbian chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.227
H-Index - 45
eISSN - 1820-7421
pISSN - 0352-5139
DOI - 10.2298/jsc210401039s
Subject(s) - chemistry , betulin , mushroom , micronucleus , triterpene , dna damage , in vitro , biochemistry , pharmacology , traditional medicine , micronucleus test , dna , biology , food science , toxicity , organic chemistry , medicine , alternative medicine , pathology
Eleven 31-methylenlanostane triterpenoids, i.e., seven 21- and four 26-oic acids, as well as a lupane triterpenoid betulin, isolated from the fruiting bodies of the mushroom Fomitopsis betulina, were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. Most of the tested compounds showed a beneficial effect by reducing DNA damage of human lymphocytes more effectively than amifostine, a radioprotective agent, used as a positive control. All the tested compounds decreased MN frequency in the concentration dependent manner, with the concentration of 2.0 ?g mL-1 being the most effective ? with increase of the concentration the activity slightly decreases. The structure?activity relationship (SAR) studies indicated that the lanostanes containing a conjugated 7,9 (11)-diene system exhibit lower activity than ?8-analogues. It was also demonstrated that the DNA protective activities within the ?8-lanostane-26-oic acid group are affected by the substitution in position 3 pattern. In the ?8 series the oxygenation at C-12 or 16 as well as 21- or 26-oic acid functionality proved beneficial for in vitro protective effect on chromosomal aberrations. Betulin exhibited the lowest protective activity, but it is still comparable to that of amifostine.