Open AccessHeadspace gas chromatography-mass spectrometry method for the determination of total cyanide concentration in water and postmortem blood samples
Author(s) -
Orhan Destanoğlu,
İsmail Ateş
Publication year - 2021
Publication title -
journal of the serbian chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.227
H-Index - 45
eISSN - 1820-7421
pISSN - 0352-5139
DOI - 10.2298/jsc200422063d
Subject(s) - cyanide , hydrogen cyanide , chemistry , chromatography , sulfuric acid , gas chromatography–mass spectrometry , mass spectrometry , gas chromatography , aqueous solution , inorganic chemistry , organic chemistry
In this study, we aimed to develop a headspace gas chromatography? ?mass spectrometry method for determining the total cyanide concentration in the forensic evidences. Total cyanide content of the samples was calculated based on the hydrogen cyanide gas concentration evaporated from the liquid sample in the headspace vial. Hexacyanoferrate(II) was used for the optimization of headspace oven temperature. We have found that iron?cyanide bonds were completely degraded after 0.2 mL of the sample was treated with 1 mL of 1 M sulfuric acid under the optimized headspace conditions where the temperature and the heating time were 120 ?C and 12.5 min, respectively. Satisfactory recovery results for both aqueous and blood samples were obtained. The method was linear in the range 0.05?10 ?g mL-1 of cyanide which was a suitable range for toxicological investigations. The proposed method was validated and applied to the post-mortem blood samples, drinking waters, and the other forensic evidences. The proposed method can easily be performed not only in the forensic laboratories, but in the related laboratories where the total cyanide analysis is a critical issue.