Open AccessNormal-phase high performance liquid chromatography of estradiol derivatives on amino- and diol-columns
Author(s) -
Marijana Ačanski
Publication year - 2003
Publication title -
journal of the serbian chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.227
H-Index - 45
eISSN - 1820-7421
pISSN - 0352-5139
DOI - 10.2298/jsc0312971a
Subject(s) - diol , chemistry , high performance liquid chromatography , chromatography , column chromatography , logarithm , phase (matter) , volume fraction , heptane , fraction (chemistry) , polar , volume (thermodynamics) , organic chemistry , thermodynamics , mathematics , mathematical analysis , physics , astronomy
The retention behavior of estradiol derivatives was studied by HPLC on chemically bonded polar stationary phases: commercially available amino and diol- columns, as a function of the heptane-propan-1-ol as the mobile phase, when the volume fraction of propan-1-ol in the binary mobile phase was low, even less than 5 %. The relationship between the logarithm of the retention constant (log k) and the logarithm of the volume fraction of propan-1-ol (?log ?) in the eluent was linear for all solutes studied. The results are discussed in terms of the solute and stationary phase properties and compared with the results of the same derivatives obtained in earlier investigations.