Open AccessSpectrophotometric determination of fluocortolone in tablets using 1,4-dihydrazinophthalazine
Author(s) -
Olivera Čudina,
Sote Vladimirov,
D. ŽivanovStakić,
Danica Agbaba
Publication year - 2000
Publication title -
journal of the serbian chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.227
H-Index - 45
eISSN - 1820-7421
pISSN - 0352-5139
DOI - 10.2298/jsc0009645c
Subject(s) - hydrazone , reagent , detection limit , chemistry , chromatography , solvent , organic chemistry
The proposed method for the determination of fluocortolone is based on the formation of a stable, yellow coloured hydrazone product with 1,4-dihydrazinophthalazine as the reagent. Heating at 85?C for 2 h was found to be necessary to ensure optimal hydrazone formation in acidified 1-propanol as the solvent. The detection limit was 1.2 ?g/ml. This method when applied for the determination of fluocortolone in pharmaceutical formulations gave precise and reproducible results.