Open AccessRedox regulation of cell cycle through nitric oxide
Author(s) -
Višnja Bogdanović,
Mihajlo Spasić
Publication year - 2008
Publication title -
hemijska industrija
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.147
H-Index - 19
eISSN - 2217-7426
pISSN - 0367-598X
DOI - 10.2298/hemind0805301b
Subject(s) - sodium nitroprusside , nitric oxide , chemistry , k562 cells , redox , superoxide dismutase , sodium ascorbate , thiol , glutathione , cell culture , cell , biochemistry , biophysics , microbiology and biotechnology , antioxidant , inorganic chemistry , biology , enzyme , genetics , ascorbic acid , organic chemistry , food science
This paper investigates the effects of sodium nitroprusside as NO donor on two cell lines in culture: transformed cells of mice fibroblasts (L929) and malignant cells of human eritroleukemia (K562). Low concentrations of NO have stimulative effect, while high concentrations have inhibitive effects on proliferation of K562 cells in a dose-dependent manner. In our experiments, by using sodium nitroprusside (SNP) as NO donor and two kinds of superoxide dismutase, Cu,Zn-SOD and Mn-SOD, we created conditions to generate several kinds of signal molecules and investigated reaction of transformed (L929) and malignant (K562) cells to dose. Results of experiments are showing that chosen parameters (amount of free thiol groups and glutathione) may be relevant in monitoring the effect of exogenous nitrate oxide and its redox descendants in different, both transformed and malignant cell lines