Open AccessBioreporter pseudomonas fluorescens HK44 immobilized in a silica matrix
Author(s) -
Josef Trögl,
Steven Ripp,
Gabriela Kuncová,
Gary S. Sayler,
Kateřina Demnerová
Publication year - 2003
Publication title -
hemijska industrija
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.147
H-Index - 19
eISSN - 2217-7426
pISSN - 0367-598X
DOI - 10.2298/hemind0312596t
Subject(s) - bioluminescence , pseudomonas fluorescens , bioreporter , chemistry , matrix (chemical analysis) , biosensor , chromatography , naphthalene , silica gel , pseudomonas , nuclear chemistry , biochemistry , bacteria , biology , organic chemistry , genetics , reporter gene , gene expression , gene
The bioluminescent bioreporter Pseudomonas fluorescens HK44, the whole cell bacterial biosensor that responds to naphthalene and its metabolites via the production of visible light, was immobilized into a silica matrix by the sol-gel technique. The bioluminescence intensities were measured in the maximum of the bioluminescence band at X = 500 nm. The immobilized cells (>105 cells per g silica matrix) produced light after induction by salicylate (cone. > 10 g/l), naphthalene and aminobenzoic acid. The bioluminescence intensities induced by 2,3-dihydroxynaphthalene 3-hydroxybenzoic acid and 4-hydroxybenzoic acid were comparable to a negative control. The cells in the silica layers on glass slides produced light in response to the presence of an inductor at least 8 months after immobilization, and >50 induction cycles. The results showed that these test slides could be used as assays for the multiple determination of water pollution
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