Open AccessImprovement of micropropagation protocol of Phalaenopsis sp. for the method of direct shoot regeneration from nodes of floral spikes
Author(s) -
Marija Marković,
Milos Tanasic,
Nevena Stojic,
Radivoje Bulatovic,
Marta Jovic,
Slobodan Vidojkovic,
Dušan Stanković
Publication year - 2012
Publication title -
glasnik šumarskog fakulteta - univerzitet u beogradu/glasnik šumarskog fakulteta
Language(s) - English
Resource type - Journals
eISSN - 2217-8600
pISSN - 0353-4537
DOI - 10.2298/gsf1206141m
Subject(s) - explant culture , phalaenopsis , micropropagation , shoot , biology , horticulture , botany , regeneration (biology) , in vitro , microbiology and biotechnology , biochemistry
This paper succesfully investigated the possibility of modification of the micropropagation protocol of Phalaenopsis sp. with an aim to simplify the procedure and reduce the costs. The obtained results show that some medium components can be succesfully omitted (coconut water, glutamine, 2-morpholinoethanesulfonic acid) and some of them (peptone) can be replaced with a cheaper constituent (soy flour) while preserving the quality of the obtained microplants. The multiplication rate was 7,6 shoots per explant after the period of 150 days of cultivation in vitro. On the same medium 60% of explants were rooted and roots were mostly well developed