Open AccessEffect of explant type, medium and soil mixture content on Dianthus deltoides L. rooting and acclimatization
Author(s) -
Marija Marković,
Marija Popović
Publication year - 2012
Publication title -
glasnik šumarskog fakulteta - univerzitet u beogradu/glasnik šumarskog fakulteta
Language(s) - English
Resource type - Journals
eISSN - 2217-8600
pISSN - 0353-4537
DOI - 10.2298/gsf1205117m
Subject(s) - explant culture , cutting , acclimatization , shoot , ramification , biology , botany , horticulture , murashige and skoog medium , dianthus , in vitro , mathematics , carnation , biochemistry , combinatorics
The possibility of developing roots and shoots on Dianthus deltoides nodal and apical cuttings on rooting media was investigated in vitro conditions. The explants developed roots and shoots successfully, and the best results were achieved on MS medium without plant hormones. Number of roots per shoot, length of roots and ramification of roots were not significantly dependent on the medium (NAA-naphthaleneacetic acid concentration), or on the type of explant used (nodal or apical cuttings). The acclimatization rate of microplants was influenced by root numbers and ramification. The transfer to soil was successful, maximum survival rates (96%) were achieved on peat - sand (4:1) mixture