Open AccessPossibility of micropropagation of dianthus giganteiformis subsp. Kladovanus (Degen) SÓO by the method of proliferation of lateral shoots
Author(s) -
Marija Marković,
Mihailo Grbić,
Ana Sindelic
Publication year - 2006
Publication title -
glasnik šumarskog fakulteta - univerzitet u beogradu/glasnik šumarskog fakulteta
Language(s) - English
Resource type - Journals
eISSN - 2217-8600
pISSN - 0353-4537
DOI - 10.2298/gsf0694171m
Subject(s) - micropropagation , dianthus , shoot , carnation , sucrose , murashige and skoog medium , botany , horticulture , agar , biology , chemistry , tissue culture , in vitro , food science , biochemistry , genetics , bacteria
The micropropagation of kladovanus carnation (Dianthus giganteiformis subsp kladovanus) was tested by the lateral shoot proliferation method. Micropropagation was performed on the medium with 1/2 MS mineral solution supplemented with 3% sucrose, 0.8% agar, 50 mg·L-1 mio-inositol, 0.025 mg·L-1 thiamin, 0.125 mg·L-1 nicotinic acid and 0.5 mg·L-1 glycine. The development of lateral shoots was induced on 6-benzyl-aminopurine (BAP) and α-naphthaleneacetic acid (NAA). The best results were achieved on the medium with 1 mg·L-1 BAP and 0.1 mg·L-1 NAA. Rooting was successful on the medium without hormones, and the percentage of acclimatisation was 83%