Open AccessProtein quality parameters and storage protein profiling of mungbean interspecific lines (Vigna radiata L. Wilczek)
Author(s) -
Vritika Jain,
Sanjeev Sharma
Publication year - 2021
Publication title -
genetika
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.24
H-Index - 15
eISSN - 1820-6069
pISSN - 0534-0012
DOI - 10.2298/gensr2103341j
Subject(s) - vigna , globulin , interspecific competition , storage protein , methionine , glutelin , tryptophan , albumin , biology , gel electrophoresis , chemistry , amino acid , biochemistry , botany , gene , immunology
Present investigation reports the protein quality parameters in mungbean x ricebean (MR) and mungbean x urdbean (MU) interspecific lines along with mungbean checks. Interspecific lines contained 138.4-230.3 mg/g total soluble proteins, 0.84-2.63 mg/g free amino acids, 3.21-7.10 mg/g methionine, 0.08-0.15 mg/g cysteine and 0.67-1.32 mg/g tryptophan. Protein fractionation studies revealed 32.6-48.3 (albumins), 153.3- 218.8 (globulins), 2.81-8.30 (prolamins) and 22.22-66.23 mg/g seed (glutelins). Among MU lines, mean albumin content was lower and globulins, prolamins and glutelins were significantly higher than that of mungbean checks. Globulins showed a positive correlation with total soluble proteins and tryptophan while albumins were negatively correlated to prolamins and positively correlated to methionine. High positive correlation was recorded between prolamins and glutelins, as well as prolamins and cysteine at 1% level of significance. Both prolamins and glutelins exhibited a negative correlation with methionine at P<0.05. Electrophoretic analysis of total proteins revealed presence of 6-11 bands in molecular weight ranging from 13-162 kDa. Cluster analysis of the resolved gel revealed 9 clusters at 95% homology with SML 2011, the most distant interspecific line among all genotypes. Overall, SML 1827 and SML 2033 were rich in total soluble proteins, free amino acids, methionine, tryptophan, albumins and globulins. The results suggest that electrophoretic profiles of mungbean interspecific lines provide valuable information with potential of being used in mungbean genetic improvement.