Open AccessDiversity and phylogeny of saffron (Crocus sativus L.) accessions based on iPBS markers
Author(s) -
Mahdi Bayat,
Reza Amirnia,
Hakan Özkan,
Aysun Gedik,
Duygu Ateş,
Bahattin Tanyulac,
Mehdi Rahimi
Publication year - 2018
Publication title -
genetika
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.24
H-Index - 15
eISSN - 1820-6069
pISSN - 0534-0012
DOI - 10.2298/gensr1801033b
Subject(s) - crocus sativus , genetic diversity , phylogenetic tree , biology , genetic distance , primer (cosmetics) , genetics , genetic variation , botany , evolutionary biology , gene , population , medicine , environmental health , chemistry , organic chemistry
This study sought to measure genetic diversity and phylogenetic structure among 196 individuals of saffron from 20 geographically separate accessions from Iran, Spain and Turkey using retrotransposon derived iPBS markers. Twenty-eight primers amplified a total of 179 polymorphic alleles with an average of 6.4 bands per primer. The average of parameters shannon's information index, genetic distance and gene diversity was 0.483, 0.286 and 0.841 respectively. Polymorphic information index ranged from 0.407 to 0.953 with an average of 0.824. Primers 2298, 2229 and 2393 with 0.953, 0.943 and 0.943 PIC respectively, identified as the most informative primers in this study. The results of phylogenetic trees showed that twenty saffron accessions were placed into four major clusters that matched with their geographical locations completely. These results are supported by principal coordinate analysis. Overall, we can confirm that iPBS markers as low cost and high efficient molecular markers are a powerful DNA fingerprinting for assessing genetic diversity and phylogenetic analysis among saffron accessions originating from different geographical regions.