Open AccessIn vitro plant regeneration of two cucumber (Cucumis sativum L.) genotypes: Effects of explant types and culture medium
Author(s) -
Stanislava Grozeva,
N. Velkov
Publication year - 2014
Publication title -
genetika
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.24
H-Index - 15
eISSN - 1820-6069
pISSN - 0534-0012
DOI - 10.2298/gensr1402485g
Subject(s) - explant culture , cucumis , hypocotyl , organogenesis , biology , cultivar , sativum , botany , regeneration (biology) , tissue culture , cotyledon , murashige and skoog medium , in vitro , horticulture , microbiology and biotechnology , gene , biochemistry
The effect of different phytohormone concentrations on callusogenesis and organogenesis in two cucumber genotypes were studied. It was established that the rate of plant regeneration depends on genotype, explant type and culture medium. Hypocotyls were found to be more responsive than cotyledons in morphogenesis. In vitro planlet-regenerants have been obtained in hypocotyls explants on culture medium with 1.0 and 2.0 mgL-1 BA for cultivar Gergana and in 1.0 and 3.0 mgL-1K-line 15B. Induction of regeneration in cotyledons were established only in cultivar Gergana on culture medium supplemented with 3.0 mgL-1 BA and in combination of 0.5 mgL-1IAA