Open AccessInduction and micropropagation potential of sugar beet haploids
Author(s) -
Nevegl,
S. Mezei,
Lazar Kovačev,
Dragana M. Vasić,
Nikola Čačić
Publication year - 2004
Publication title -
genetika
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.24
H-Index - 15
eISSN - 1820-6069
pISSN - 0534-0012
DOI - 10.2298/gensr0403187n
Subject(s) - micropropagation , biology , sugar beet , ploidy , ovule , horticulture , botany , genotype , axillary bud , tissue culture , in vitro , genetics , gene , pollen
The aim of research was obtaining sugar beet haploids via gyno-genesis and their micropropagation. Haploids were obtained by ovule culture from fourteen diploid, monogerm, fertile genotypes. On the tested nutrient media genotypes exhibited different gynogenic potential. Eight haploid plant were chosen for further investigation and after development of first leaves put on micropropagation medium. The presence of cyto-kinin in medium stimulated development of axillary buds, while in some genotypes adventitious buds developed as well. Multiplication rate was not consistent, although number of developed plants grew after each sub-cultivation. Differences in plant multiplication started to differ after four subcultures. By testing of differences between correlation coefficients, i.e. multiplication rate during six subcultivations, it was determined that they significantly differ between tested genotypes