Open AccessUltrasonic-enchanced compound-enzymes-assisted extraction of polysaccharides from Cornus officinalis
Author(s) -
Qidong You,
Xin Yin,
Hong Xu
Publication year - 2015
Publication title -
chemical industry and chemical engineering quarterly
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.189
H-Index - 26
eISSN - 2217-7434
pISSN - 1451-9372
DOI - 10.2298/ciceq140130015y
Subject(s) - response surface methodology , polysaccharide , extraction (chemistry) , yield (engineering) , dpph , ultrasonic sensor , chromatography , officinalis , chemistry , botany , materials science , organic chemistry , biology , antioxidant , physics , acoustics , metallurgy
Process of ultrasonic-enchanced compound-enzymes-assisted extraction of polysaccharides from the fruit of Cornus officinalis was optimized by response surface methodology (RSM). The influence of three different factors on the yield of Cornus officinalis polysaccharides (COP) was studied. Results showed that the optimal conditions were extraction temperature 49.6°C, ultrasound time 40.41 min, and ultrasound power 308.07 W. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and were analyzed by analysis of variance (ANOVA). The 3-D response surface plot and the contour plot derived from the mathematical models were applied to determine the optimal conditions. Under modificated conditions, the experimental yield of polysaccharides was 11.02 ± 0.41%, which was well in agreement with the value predicted by the model. A good 1,1-diphenyl-2-picryldydrazyl (DPPH) radical scavenging activity of COP extracted by ultrasonic-enchanced compound-enzymes-assisted extraction was observed