Open AccessOptimization of inulinase production using copra waste by K. marxianus var. marxianus
Author(s) -
M. Dilipkumar,
M. Rajasimman,
Natarajan Rajamohan
Publication year - 2010
Publication title -
chemical industry and chemical engineering quarterly
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.189
H-Index - 26
eISSN - 2217-7434
pISSN - 1451-9372
DOI - 10.2298/ciceq091211032d
Subject(s) - inulinase , copra , corn steep liquor , food science , chemistry , kluyveromyces marxianus , response surface methodology , plackett–burman design , fermentation , pulp and paper industry , microbiology and biotechnology , chromatography , biochemistry , biology , yeast , engineering , saccharomyces cerevisiae
Kluyveromyces marxianus var marxianus was found to secrete a large amount of extracellular inulinase in to the medium. The optimization of inulinase production using copra waste as carbon source was performed with statistical methodology based on experimental designs. The screening of eighteen nutrients for their influence on inulinase production was achieved using a Plackett-Burman design. Corn steep liquor, (NH4)2SO4, ZnSO4.7H2O, K2HPO4 and urea were selected based on their positive influence on inulinase production. The selected components were optimized using Response Surface Methodology (RSM). The optimum conditions are: corn steep liquor - 0.0560 (g/gds), (NH4)2SO4 - 0.0084 (g/gds), ZnSO4.7H2O - 0.0254 (g/gds), K2HPO4 - 0.0037 (g/gds) and urea - 0.02147 (g/gds). These conditions were validated experimentally which revealed an enhanced inulinase yield of 372 U/gds.