Open AccessIonic liquids as (co)solvents for enzymatic reactions
Author(s) -
Muzafera Paljevac,
Maja Habulin,
Željko Knez
Publication year - 2006
Publication title -
chemical industry and chemical engineering quarterly
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.189
H-Index - 26
eISSN - 2217-7434
pISSN - 1451-9372
DOI - 10.2298/ciceq0603181p
Subject(s) - ionic liquid , hexafluorophosphate , tetrafluoroborate , chemistry , lipase , catalysis , hydrolysis , organic chemistry , enzyme
Ionic liquids are low melting point salts that represent an excitnq new class of reaction solvents. Many reactions show advantages when carried out in ionic liquids, either with regard to enhanced reaction rates, improved selectivity, or easier reuse of catalysts. To ascertain the influence of ionic liquids on the enzyme activity three different ionic liquids, 1-butyl-3-methylimidazolium chloride ([bmim] [Cl]), 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim] [PF6]) and 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF4]) were synthesized and investigated as potential media for the hydrolysis of carboxymethyl cellulose, catalyzed by non-immobilized cellulase from Humicola insolens (Celluzyme 0,7T) and for ester synthesis, catalyzed by immobilized lipase from Rhyomucor miehei (Lipozyme RM IM). Enzyme-catalyzed reactions were performed in a batch stirred reactor at atmospheric pressure. Celluzyme 0.7T showed better activity in hydrophobic ionic liquid ([bmim] [PF6]), as compared to hydrophilic ionic liquid ([bmim] [BF4]). In the case of Lipozyme RM IM, the synthetic activity of the enzyme was strongly reduced by incubating the enzyme in ionic liquids.Paper presented at the 1st South-East European congress of chemical engineering, Belgrade, September 25-28, 2005Jonske tečnosti imaju niske temperature mržnjenja i kao takve predstavljaju novu klasu rastvarača koji se mogu iskoristiti u mnogim reakcijama. Očigledne su prednosti kada se hemijska reakcija izvodi uz prisustvo jonskih tečnosti bilo kod ubrzanja brzine hemijske reakcije, povećane selektivnosti ili jednostavnije ponovne upotrebe katalizatora. U cilju utvrđivanja uticaja jonskih tečnosti na aktivnost enzima, sintetizovane su i ispitane tri različite tečnosti, l-butil-3-meilimidazolijum hlorid ([bmim][CI]) 1-butil-3-metilimidazolijum heksafluorofosfat ([bmim][PF6]) i 1-butil-3-metilimidazolijum tetrafluoroborat ([bmim][BF4]) kao potencijalni rastvarači u reakcijama hidrolize karboksimetil celuloze koja je katalizovana neimobilisanom celulazom iz Humicola insolens (Celluzyme 0,7T) i sintezama estara katalizovanim imobilisanom lipazom iz Rhizomucor miehei (Lipozvme RM IM). Enzimski katalizovane reakcije su izvedene u šaržnom reaktoru pod atmosferskim pritiskom. Pokazalo se da Celluzyme 0,7T ima veću aktivnost u hidrofobnoj jonskoj tečnosti [bmim][PF6]), u odnosu na hidrofilnu ([bmim][BF4]). U slučaju Lipozyme RM IM, aktivnost enzima je značajno smanjena inkubacijom enzima u jonskoj tečnosti