Open AccessThe development pattern of IGF-1 (insulin- like growth factor-1) protein expression in breast muscle of broiler chickens
Author(s) -
Zdenko Kanački,
Slobodan Stojanović,
Gordana Ušćebrka,
Dragan Žikić
Publication year - 2012
Publication title -
biotechnology in animal husbandry/biotechnology in animal husbandry
Language(s) - English
Resource type - Journals
eISSN - 2217-7140
pISSN - 1450-9156
DOI - 10.2298/bah1204797k
Subject(s) - broiler , biology , embryogenesis , insulin like growth factor , autocrine signalling , embryo , medicine , endocrinology , paracrine signalling , andrology , growth factor , microbiology and biotechnology , cell culture , genetics , zoology , receptor
It is proved that the protein expression of IGF-1 is detected in several tissues including liver, brain, eye, lung, pancreas and muscle. This growth factor exerts autocrine and paracrine effect during embryonic and postnatal development and is involved in muscle development. The aim of this study was to determine the level of protein expression of IGF-1 in breast muscle during embryonic and postnatal development of broiler chickens. For the experiment were used fertilized eggs and broiler chickens of heavy hybrid "Ross 308", originating from parent flock at the age of 54 weeks. Samples of breast muscle were taken from embryos during the 15th and 19th day of embryonic development and 3rd and 21st day of postnatal development from 5 birds in each age category. From all of the embryos and chickens the right side of the brest muscles were individually sampled. The muscle tissue was macerated and then homogenized in buffer with protease inhibitors. After that immunoprecipitation, electrophoresis and transfer the Western blot analysis were performed on all samples. For visualization of proteins the membranes were incubated in chemiluminescent substrate. The results indicate statistically significant increase in protein expression of IGF-1 in breast muscle on the 19th day of embryonic development and 3rd of postnatal development in compared to 15th day of embryonic development (p<0,05 compared to 19th day of embryonic development and p<0,01 compared to the 3rd day of postnatal development). After the 3rd day of postnatal development the protein expression was at the same level until the 21st day of postnatal development.