Open AccessEvidences reveal that cattle and buffalo evolutionary derived from the same ancestor based on cytogenetic and molecular markers
Author(s) -
Salah Abdel-Rahman
Publication year - 2006
Publication title -
biotechnology in animal husbandry/biotechnology in animal husbandry
Language(s) - English
Resource type - Journals
eISSN - 2217-7140
pISSN - 1450-9156
DOI - 10.2298/bah0604001a
Subject(s) - restriction fragment length polymorphism , biology , cytochrome b , mitochondrial dna , genetics , polymerase chain reaction , gene , microbiology and biotechnology
Muscle-DNA from cattle and buffalo was extracted to amplify the mitochondrial DNA segment (cytochrome b gene) and the gene encoding species-specific repeat (SSR) region. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and SSR techniques were used to identify of species origin. Restriction analysis of PCR-RFLP of the mitochondrial cytochrome b segment and SSR analysis showed no differences between cattle and buffalo. Where, the fragment length (bp) generated by AluI PCR-RFLP were 190, 169 and PCR amplification size of the gene encoding SSR region was 603 bp in both cattle and buffalo. Consequently, finding from this study could be revealed that cattle and buffalo are evolutionary derived from the same ancestor.