Open AccessPCR amplification of species-specific repeat for meat DNA identification via genetic markers in cattle & sheep
Author(s) -
M. A. Ahmed
Publication year - 2005
Publication title -
biotechnology in animal husbandry/biotechnology in animal husbandry
Language(s) - English
Resource type - Journals
eISSN - 2217-7140
pISSN - 1450-9156
DOI - 10.2298/bah0502001a
Subject(s) - biology , polymerase chain reaction , genetic marker , beef cattle , gene , veterinary medicine , genetics , medicine
The designed and evaluated four assays based upon PCR amplification of species-specific repeat (SSR) for detection, identification and authentication of cattle and sheep on the DNA level. SSR primers were applied in the polymerase chain reaction (PCR), the products has been used for the specific identification of cattle and sheep meat. PCR amplification size of the gene encoding SSR region in cattle and sheep meat was 603 bp and 374 bp respectively. The results showed that SSR analysis produced a pattern that allowed a direct identification of cattle and sheep meat in raw and heat-treated samples. Also the results showed that SSR analysis provided with a rapid and effective method to detect the meat species and it could be easily identified and authenticated. In addition, the SSR PCR methods, as species-specific, are highly sensitive and will improve the detection limits for DNA sequences derived from these species. Also indirect application is the detection of disease cow (cattle) lunacy by PCR methods for authentication of cow (cattle) meat from origin country.