Open AccessMultiplication of HVT FC-126 (Herpesvirus turkey) virus in the kidney cell lines of no avian origin
Author(s) -
Bratko Filipič,
Željko Gottstein,
Srećko Sladoljev,
Avrelija Cencič,
Simon Koren,
Irena Ciglar Grozdanić,
Hrvoje Mazija
Publication year - 2010
Publication title -
acta veterinaria
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.308
H-Index - 17
eISSN - 1820-7448
pISSN - 0567-8315
DOI - 10.2298/avb1004381f
Subject(s) - titer , cell culture , virus , biology , virology , inoculation , kidney , microbiology and biotechnology , immunology , genetics , endocrinology
The presented experiments were aimed to cultivate and multiplicate HVT FC-126 in the PLA (Adult pig kidney) and GL-4 (Gerbil kidney) cell lines. Two different HVT FC-126 vaccine strains were used: Marikal SPF (Veterina d.o.o., Croatia) and Lyomarex (Merial, USA). They were adapted to the PLA and GL-4 cell lines. After adaptation, they were titrated on PLA (TCID50 24.23) and GL-4 (TCID50 24.96). On both cell lines they show similar CPE (cytophatic effect). The difference between them was detected using Real Time PCR, which was also positive by agarose gel analysis for the virus contained in Lyomarex, but not in the Marikal SPF. It can be concluded that both cell lines are sensitive to HVT FC-126 and the virus can be multiplied in high titers though much lower than in the calf intestinal epithelial cell line (CIEB) cells (TCID50 27.93)