Qualitative and quantitative parameters of swine cell immunity | Zendy

Maja Popović | Zendy; H. Valpotic | Zendy; Kardum-Paro Marijana | Zendy; Željko Pavičić | Zendy; Vijtiuk Nada | Zendy; Popovic Iva | Zendy; Resanovic Ruzica | Zendy; I. Valpotić | Zendy

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Qualitative and quantitative parameters of swine cell immunity

Author(s) -

Maja Popović,

H. Valpotic,

Kardum-Paro Marijana,

Željko Pavičić,

Vijtiuk Nada,

Popovic Iva,

Resanovic Ruzica,

I. Valpotić

Publication year - 2008

Publication title -

acta veterinaria

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.308

H-Index - 17

eISSN - 1820-7448

pISSN - 0567-8315

DOI - 10.2298/avb0803149p

Subject(s) - flow cytometry , immunophenotyping , cytometry , population , cell counting , cd3 , biology , cell , immunology , veterinary medicine , medicine , immune system , cd8 , biochemistry , environmental health , cell cycle

Flow cytometry is a modern analytic method for qualitative and quantitive analyses of procaryotic and eukaryotic cells. Classical analytical methods examine one characteristic as an average for an examined cell population. Flow cytometry simultaneously measures several biochemical and physical parameters of every single cell in a heterogenic cell sample. Flow cytometry is routinely used all over the world in professional laboratories in veterinary medicine. For that purpose on the Veterinary Faculty of University in Zagreb, a machine for flow cytometry was standardized for use in veterinary medicine, especially for immunophenotyping of haemopoetic cells of domestic animals. In this manuscript we showed the standardisation method on a swine model, on samples of peripheral blood for the immunophenotypification of CD45+, CD3+ and CD21+ cells.

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