Open AccessEfects of ethanol on electroencephalographic and behavioral signs of metaphit-induced audiogenic seizure
Author(s) -
Olivera Stanojlović,
Bojana Petrović,
Dragan Hrnčić,
Dušan Mladenović,
Aleksandra Rašić,
Veselinka Šušić
Publication year - 2008
Publication title -
acta veterinaria
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.308
H-Index - 17
eISSN - 1820-7448
pISSN - 0567-8315
DOI - 10.2298/avb0803111s
Subject(s) - ethanol , reflex epilepsy , anesthesia , stimulation , electroencephalography , saline , epilepsy , spike and wave , psychology , reflex , chemistry , medicine , audiology , neuroscience , biochemistry
The goal of the experiment was to give an answer to the question whether the simultaneous action of metaphit and audiogenic stimulation, which together lead to generalized reflex seizure in rodents, could be modified by ethanol. The rats divided in four groups received (i.p.): saline; metaphit (10 mg/kg); metaphit (10 mg/kg) + ethanol (2 g/kg); and ethanol (2 g/kg). Ethanol was administered to the metaphit-treated animals which had displayed seizures in the first eight tests. Audiogenic stimulation was applied at hourly intervals starting from the first hour after giving the metaphit injection throughout 16 hours of the experiment. For EEG recordings, three gold-plated electrodes were implanted into the rat skull. Metaphit led to hypersynchrounus epileptiform activity which forms polyspikes and spike-wave complexes. Behavior was represented by established grades of motor seizures. It was noticed that ethanol significantly decreased EEG signs of seizure, reduced the frequency as the amplitude of the waves increased (dominant ones were and ). Ethanol completely blocked all the manifestations of the convulsive behavior of metaphit-treated animals. The results of this experiment suggest that ethanol inhibits behavioral and modifies EEG signs of the metaphit induced audiogenic generalized epilepsy.