Open AccessPromene aktivnosti enzima superoksid dizmutaze, glutathione reduktaze, redukovanog glutationa i količine malonil dialdehida nakon akutnog trovanja aluminijumom
Author(s) -
Jovica Milovanović,
Aleksandra Milovanović,
Ljubica Konstantinović,
Vesna Đorđević,
P. Stanković,
Bruna Babic,
N. Arsović,
Vanja Dukić
Publication year - 2007
Publication title -
acta veterinaria
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.308
H-Index - 17
eISSN - 1820-7448
pISSN - 0567-8315
DOI - 10.2298/avb0701059m
Subject(s) - glutathione , glutathione reductase , lipid peroxidation , chemistry , antioxidant , superoxide dismutase , saline , reductase , biochemistry , enzyme , endocrinology , biology , glutathione peroxidase
The important and increasing application of aluminum motivated us to determine whether and in what way does the antioxidative status of erythrocytes in aluminum acute poisoned animals change by studying the metabolism of superoxid dismutase (SOD), glutathione reductase (GR), reduced glutathione (GSH) and malonil dialdehyde (MDA). The experiment involved desert mice (gerbils) who received intraperitonally an aluminum chloride solution at a dose of 3.7 g/kg BM. The experimental groups of animals were sacrified after 24, 48, 72 and 96 hours from the beginning of the experiment by cardial punction or by punction of the abdominal artery. To the control group saline solution was applied. It has been noticed that during the course of the experiment SOD activity increased. We assume this represents a defense mechanism of the organism to increased quantities of free radicals. Further, aluminum considerably reduces the glutathione reductive system capacity affecting its enzymatic component, glutathione reductase which leads to a further decrease in reduced glutathione. The lipid peroxidation index expressed through MDA as an indicator of membrane status remains unchanged. This could mean that malonil dialdehyde is measured in erythrocytes not jet structurally damaged.