Open AccessCharacterization of transcription factors binding to-120 GATA motif of rat βbminy-globin promoter
Author(s) -
T Pavlovic Sonja,
Tatjana Mitrović,
Teodora Karan-Djurašević,
T Nikcevic Gordana
Publication year - 2005
Publication title -
acta veterinaria
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.308
H-Index - 17
eISSN - 1820-7448
pISSN - 0567-8315
DOI - 10.2298/avb0506347p
Subject(s) - gata transcription factor , electrophoretic mobility shift assay , transcription factor , microbiology and biotechnology , promoter , gata2 , transcription (linguistics) , gene , globin , biology , e box , enhancer , chemistry , gene expression , genetics , linguistics , philosophy
The aim of this study was to elucidate the regulation of rat adult βbminy-globin gene transcription. We used DNasel foot printing, gel mobility shift and super shift assays to characterize transcription factors involved in this regulation. In this study we analyzed GATA motif at-120 bp in the distal promoter of βbminy-globin gene. Footprint analysis revealed the binding of nuclear factors from MEL cells to the GATA motif. By using gel mobility shift assay two protein complexes were detected. The faster migrating complex was erythroid-specific and more abundant in differentiating MEL cells. Competition experiments with GATA-1 oligonucleotides and GATA-1 protein antibodies confirmed binding of GATA-1 transcription factor to GATA motif at - 120 bp regulation of rat adult βbminy-globin gene