Open AccessCytogenetic analysis of the effects of epinephrine on cultured human lymphocytes
Author(s) -
J Djelic Ninoslav,
J Djelic Dijana,
M Potparevic Biljana,
M Potparevic Biljana,
Piotr Łada
Publication year - 2003
Publication title -
acta veterinaria
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.308
H-Index - 17
eISSN - 1820-7448
pISSN - 0567-8315
DOI - 10.2298/avb0303113d
Subject(s) - epinephrine , mitotic index , catechol , in vitro , chemistry , catecholamine , mitosis , endocrinology , medicine , pharmacology , biochemistry , biology , genetics
Epinephrine can modulate mitotic activity of normal and malignant cells and exhibit genotoxic potential in some test-systems. It is assumed that metabolic conversion of phenolic groups in the catechol ring of epinephrine leads to the formation of reactive derivatives and superoxide anions capable of damaging cellular molecules including DNA. The aim of the present study was to evaluate the cytotoxic and genotoxic effects of epinephrine on human peripheral blood lymphocytes in vitro. The lowest concentration of epinephrine used in these experiments (5x10-10 M) was calculated to be in the range of the physiological blood level of epinephrine in humans. Three experimental concentrations corresponded to minimal (2x10-7 M), average (10-6 M) and maximal (5x10-6 M) therapeutic doses in human medicine. In addition, the highest concentrations exceeded the maximal therapeutic dose 10-fold (5x10-5 M) and 30-fold (1.5x10-4 M), respectively. On the basis of the results obtained it can be concluded that epinephrine had no influence on the appearance of chromosome aberrations under the described experimental conditions. However, mitotic index was significantly lower in cultures treated with the three highest concentrations of epinephrine used in this investigation