Open AccessIdentification and characterization of rat β-DRE binding factors involved in erythroid-specific induction of transcription
Author(s) -
T Pavlovic Sonja,
R Kovacevic-Grujicic Natasa,
Tatjana Mitrović
Publication year - 2003
Publication title -
acta veterinaria
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.308
H-Index - 17
eISSN - 1820-7448
pISSN - 0567-8315
DOI - 10.2298/avb0303067p
Subject(s) - promoter , microbiology and biotechnology , transcription factor , biology , transcription (linguistics) , electrophoretic mobility shift assay , globin , gene , dna binding protein , response element , gene expression , genetics , philosophy , linguistics
We have identified and characterized a DNA-binding activity with specificity for the β-globin direct repeat element (βDRE) from rat βbminy-globin promoter, an evolutionarily conserved transcriptional regulatory element in mammalian adult β-globin promoters. We have also confirmed that the βDRE contributed to the transcriptional inducibility of rat βbminy-globin gene in rat erythroleukemia (REL) cells. By using gel mobility shift and South-Western blot competition studies we have shown that 60 kD erythroid-specific transcription factor and 35 kD and 20 kD ubiquitous factors from rat erythroleukemia cells specifically bind to the βDRE from the rat βbminy-globin promoter. Additionally, a significant increase in the quantity of 60 kD transcription factor was observed upon erythroid differentiation of REL cells