Open AccessThe induction of inflammation in mouse tails with Aspergillus oryzae protease and the inhibitory effects of synthetic and natural sub stances in vitro
Author(s) -
V. Šutiak,
Marián Korének,
Irena Šutiaková,
Beáta Korénekova,
Anna Sobeková,
Ladislav Vaško,
J Neuschl
Publication year - 2002
Publication title -
acta veterinaria
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.308
H-Index - 17
eISSN - 1820-7448
pISSN - 0567-8315
DOI - 10.2298/avb0206345s
Subject(s) - aspergillus oryzae , chemistry , in vitro , protease , saline , inflammation , inhibitory postsynaptic potential , pharmacology , incubation , enzyme , biochemistry , biology , endocrinology , immunology
Four groups of male mice were injected with doses from 0.005 to 1.0 mg of protease ex Aspergillus oryzae (PAO) in 20.0 (l of a 0.9 % physiological saline solution (PSS) per mouse s.c. into their tails. The control mice received 20.0 (l of 0.9% PSS only. The inflammation of the tails was measured volumetrically before injection and 1, 2, and 3 days afterwards. The clinical responses of the mice to PAO were observed up to 30 days. The development of inflammation corresponded well with the quantity of enzyme used. The data obtained indicated the smallest dose (0.005 mg) of PAO induced oedemization of the tail (P<0.01) and more significant and longer lasting tail changes were observed at the higher doses. For inhibitory studies in vitro, we used the following conditions: 450 (gr PAO. 0.1 ml(-1), 20 mmol. 0.1 ml(-1) chromogenic substrate Suc-(Gly)2-Phe-NAn and Tris/HCI buffer in the volume of 0.7ml. Incubation was carried out at 37 °C for 15 min. The results obtained demonstrated that all three synthetic compounds (11 = (Ala)2-Leu-NH-EtPh, I 2 = Suc-(Ala)2 -Pro-NH-EtPh and I 3 = Glt-(Ala)2-Pro-NH-EtPh) exhibited inhibitory effects and four natural preparations of tannin (Tanin plv., Tanifarm plv.sol. a.u.v., Farmatan cps. a.u.v., and Pycnogel tbl) had even more effective inhibitory action (P<0.01) on PAO