Open AccessAdsorption of cellulases onto sugar beet shreds and modeling of the experimental data
Author(s) -
Darjana Ž. Ivetić,
Radovan Omorjan,
Mirjana G. Antov
Publication year - 2014
Publication title -
acta periodica technologica/acta periodica technologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.134
H-Index - 15
eISSN - 2406-095X
pISSN - 1450-7188
DOI - 10.2298/apt1445119i
Subject(s) - cellulase , adsorption , chemistry , sugar beet , substrate (aquarium) , sugar , nuclear chemistry , chromatography , cellulose , pulp and paper industry , biochemistry , organic chemistry , horticulture , biology , engineering , ecology
This study investigated the adsorption of cellulases onto sugar beet shreds. The experiments were carried out using untreated, as well as dried and not dried dilute acid and steam pretreated sugar beet shreds at different initial enzyme loads. Both dilute acid and steam pretreatment were beneficial in respect of cellulases adsorption providing 8 and 9 times higher amounts of adsorbed proteins, respectively, in comparison to the results obtained with the untreated substrate. Although the use of higher solids load enabled by drying of pretreated substrates, could be beneficial for process productivity, at the same time it decreases the adsorption of enzymes. The obtained experimental data were fitted to five adsorption models, and the Langmuir model having the lowest residual sum of squares was used for the determination of adsorption parameters which were used to calculate the strength of cellulases binding to the substrates.[Projekat Ministarstva nauke Republike Srbije, br. TR 31002