Open AccessThe effect of erythropoietin on alanine aminotransferase during ischemia reperfusion injury in rats
Author(s) -
Constantinos Tsompos,
Constantinos Panoulis,
K. Ïtutouzas,
George Zografos,
Αpostolos Papalois
Publication year - 2015
Publication title -
acta chirurgica iugoslavica
Language(s) - English
Resource type - Journals
eISSN - 2406-0887
pISSN - 0354-950X
DOI - 10.2298/aci1502033t
Subject(s) - erythropoietin , medicine , alanine aminotransferase , reperfusion injury , ischemia , alanine transaminase , endocrinology , alanine , pharmacology , biochemistry , chemistry , amino acid
The aim of this experiment study was the erythropoietin testing, on the rat model and particularly on ischemia reperfusion protocol. The benefit or the non effect of that molecule was studied biochemically on blood alanine aminotransferase. Material and methods 40 rats were used of mean weight 247,7 gr. Alanine aminotrans-ferase was measured at these time points: on 60 min after reperfusion (groups A and C), and on 120 min after reperfusion (groups B and D), A and B without but C and D with erythropoietin administration. Results were that 1) erythropoietin administration increased significantly the ALT by 15.2 IU/L [-0.6168546 IU - 31.01685 IU/L] (P= 0.0591), in accordance also with paired t-test (P=0.0480), 2) reperfusion time increased non significantly the ALT by 3.4 IU/L [-13.1482 IU - 19.9482 IU/L] (P= 0.6798), in accordance also with paired t-test (P= 0.5994), and 3) interaction with erythropoietin administration and reperfusion time increased non significantly the ALT levels by 3.581818 IU/L [-6.350404 IU-13.51404 IU/L] (P= 0.4698). Conclusions are that erythropoietin administration, reperfusion time and their interaction have non significant short-term increasing effects on alanine aminotransferase.