Open AccessEffects of mistletoe extract on markers of platelet activation and aggregation
Author(s) -
Tatjana Srdić-Rajić,
Aleksandra Konić-Ristić,
Nevena Tišma-Miletić,
Nevena Kardum,
Danijel Galun,
Jelena Sikimić,
Marija Glibetić,
Miroslav Milićević
Publication year - 2015
Publication title -
acta chirurgica iugoslavica
Language(s) - English
Resource type - Journals
eISSN - 2406-0887
pISSN - 0354-950X
DOI - 10.2298/aci1502005s
Subject(s) - viscum album , medicine , flow cytometry , loranthaceae , monocyte , platelet activation , angiogenesis , agonist , pharmacology , platelet , stimulation , apoptosis , cytotoxic t cell , tumor necrosis factor alpha , immunology , cancer research , receptor , traditional medicine , biology , biochemistry , in vitro , botany
BACKGROUND: Viscum album preparations are extensively used as complementary therapy in cancer and are shown to exert antitumor activities which involve the cytotoxic properties, induction of apoptosis, inhibition of angiogenesis and several other immunomodulatory mechanisms. AIM: The aim of this study was to investigate the effects of mistletoe extract on platelet as well as monocyte functions, as an important factors in immunomodulation of cancers metastatic potencial and angiogenesis in tumors. METHODS: The effect of different concentrations of mistletoe extract on agonist-induced platelet activation markers and their aggregation with leukocytes was examined in the blood of healthy subjects (n=6) using flow cytometry. Effects on LPS -induced activation markers was determined in the blood of healthy subjects as well as on THP-1 cell line using an ELISA essays and flow cytometry. RESULTS: Mistletoe extract significantly inhibited agonist induced P selectin expression and platelet-monocytes aggregation. Additionally, mistletoe extract exerts anti-tumor effect through the stimulation of TNF-? production in LPS induced monocytes activation. CONCLUSION: Obtained data demonstrate that mistletoe extract was effective in modulating platelet and monocyte functions, as a part of pleiotropic anticancer effect.