Open AccessAll-trans retinoic acid prevents oxidative stress-mediated cellular senescence via upregulation of insulin-like growth factor binding protein-6 in normal human epidermal keratinocytes
Author(s) -
Heekyung Choi,
Jin Lee Young,
Nok Park Hyun,
Jin Nam,
Won-Seok Park,
Chang Lee Seok,
Beom Kim Joon
Publication year - 2021
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs210615036c
Subject(s) - senescence , retinoic acid , microbiology and biotechnology , growth factor , cell growth , downregulation and upregulation , microarray analysis techniques , biology , reactive oxygen species , oxidative stress , chemistry , gene expression , cell culture , biochemistry , gene , genetics , receptor
All-trans retinoic acid (ATRA) influences cellular proliferation and differentiation but its mechanisms of action are not understood in keratinocytes. To investigate the potential mechanisms of action of ATRA in keratinocytes, microarray analysis of ATRA-treated normal human epidermal keratinocytes (NHEKs) was performed. Based on microarray data, we focused on insulin-like growth factor binding protein-6 (IGFBP-6), which is known to inhibit cellular senescence but has not been previously investigated in the context of ATRA-induced signaling in NHEKs. We verified that ATRA significantly increased IGFBP-6 gene and protein expression in NHEKs. Next, the effects of ATRA and IGFBP-6 on cell proliferation and senescence in H2O2-treated NHEKs were examined. IGFBP-6 was knocked-down using siRNA or overexpressed using pCMV-IGFBP-6. Cellular proliferation was observed using the bromodeoxyuridine (BrdU) incorporation assay. Cellular senescence was determined by monitoring SA-?-Gal staining and p21 expression. When IGFBP-6 was knocked down, cellular proliferation was inhibited, and the cellular senescence markers were increased. IGFBP-6 overexpression or ATRA treatment of H2O2-treated NHEKs rescued these effects. Taken together, our results suggest that ATRA prevents premature senescence-related skin damage at least in part by increasing IGFBP-6 expression, as shown herein in reactive oxygen species (ROS)-stimulated NHEKs.