Open AccessImmunohistochemical analysis of the arterial supply and mast cells of the trigeminal ganglion
Author(s) -
Aleksandar Mircic Lj.,
B Aleksandar Malikovic,
V Bojan Stimec,
Gordana Milosavljević,
B Dejan Cetkovic,
Marko Dožić,
S Jelena Boljanovic,
Jelena Djokovic Dj.,
V Mila Cetkovic
Publication year - 2021
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs210319016m
Subject(s) - tryptase , degranulation , mast cell , trigeminal ganglion , trigeminal neuralgia , pathology , immunostaining , ganglion , microvessel , chymase , immunohistochemistry , cd34 , anatomy , medicine , biology , microbiology and biotechnology , immunology , neuroscience , sensory system , receptor , stem cell
The aim of this study was to quantify the distribution of microvessels and mast cells in all three parts of the trigeminal ganglion (TG). Statistical analyses were applied to investigate possible micromorphological regional differences in their density. Five serially sectioned human TGs were prepared for CD34 and mast cell tryptase immunostaining. The following quantifications were performed in microscopic fields of three parts of the TG: microvessel density (MVD), mast cell density (MCD) and ganglionic cell count. The density of CD34-positive microvessels was not significantly different in any of the three observed parts of the TG. The distribution of neurons showed no significant statistical difference in three parts of the TG. There was no difference in the density of tryptase-positive mast cells within the TG, but there was an abundant presence of mast cells in the periganglionic dural and subdural tissues, a finding hitherto not reported. We can say that there is a homogenous vascular pattern within the TG which excludes local predominance in pathogenesis of trigeminal neuralgia. Second, and more important, the finding of peri-trigeminal mast cells indicates their important role in migraine pain and confirms their degranulation as the main therapeutic goal for this condition.