Open AccessProthrombin expression in cancer-derived cell lines
Author(s) -
Sofija Dunjić,
Marija Cumbo,
Maja Gvozdenov,
Branko Tomić,
Iva Pruner,
Dragica Radojković,
Veljko Đorđević
Publication year - 2019
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs180829046d
Subject(s) - colorectal cancer , cancer , thrombin , cell culture , cancer research , adenocarcinoma , cancer cell , western blot , biology , cell , medicine , oncology , genetics , gene , platelet
The link between thrombotic disorders and cancer has been known for over 150 years, although the precise mechanism of this relationship has not yet been resolved. Current data show that thrombin has a significant role in cancer metabolism, invasiveness, adhesion and survival. However, data regarding the expression of the thrombin precursor prothrombin in various cancer cell lines are scarce. Therefore, it was our objective to determine whether common cancer-derived cell lines (Caco-2, MCF-7, SK-BR-3, U-87 and U-251) express prothrombin. The prothrombin RNA expression level was assessed by qPCR, and the presence of prothrombin was analyzed by Western blot analysis. Our results show that Caco-2 cells originating from colorectal adenocarcinoma express prothrombin, whereas other analyzed cell lines do not. Our results provide a background for further research into the role of (pro)thrombin in cancer etiopathology.