Open AccessIn vivo examination of the effects of hydroxycinnamic acid on xenobiotic metabolizing and antioxidant enzymes
Author(s) -
Aslı Semiz,
Gurbet CelikTurgut,
Serdar Karakurt,
Hakan Akça,
Şevki Arslan,
Orhan Adalı,
Alaattin Sen
Publication year - 2017
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs160208083s
Subject(s) - antioxidant , chemistry , catalase , glutathione , glutathione peroxidase , biochemistry , enzyme , in vivo , nad+ kinase , pharmacology , cyp1a2 , biology , cytochrome p450 , microbiology and biotechnology
In the last decade, hydroxycinnamic acids (HCA) have gained increasing attention from researchers due to their antioxidant potential. The aim of this study was to examine in detail the impact of dietary HCA on particular types of P450 and also selected phase II and antioxidant enzymes in Wistar rat. HCA (10 mM/kg/day, i.p.) was administered for ten continuous days. Examination of the activities and mRNA and protein levels revealed that CYP2B, 2C6 and 3A enzyme activities were not altered significantly, with Western blot and qRT-PCR results corroborating this result. While treatment with HCA led to a significant reduction in CYP1A1/CYP1A2-associated enzyme activities, CYP1A1 protein, and mRNA levels were found to be unchanged. Aromatase (CYP19) activity, as well as protein and mRNA levels, were significantly reduced with HCA treatment. On the other hand, the NAD(P)H:quinone oxidoreductase 1 (NQO1), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferases (GSTs) activities were increased significantly. Also, HCA treatment significantly increased the GST-mu and GST-theta mRNA levels. These observations may be of importance given the potential use of HCA as a chemopreventive and as an anticancer agent