Open AccessMiR-139-3p is related to left ventricular hypertrophy and cardiomyocyte apoptosis in two-kidney one-clip hypertensive rats
Author(s) -
Xianghong Yang,
Hui Yu,
Hai Hu,
Jianwei Yue,
Xiaojuan Tian,
Cong Wang,
Yan Xi,
Gang Sun,
Zhanli Wang
Publication year - 2015
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs140820008y
Subject(s) - microrna , downregulation and upregulation , apoptosis , muscle hypertrophy , microarray , cardiac hypertrophy , left ventricular hypertrophy , gene expression , kidney , pathological , microarray analysis techniques , medicine , real time polymerase chain reaction , gene , biology , microbiology and biotechnology , blood pressure , genetics
MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression in many physiological and pathological processes. Previous studies have reported the role of miR-139-3p in cancer. However, its specific roles and functions in the heart undergoing hypertrophy have yet to be fully elucidated. In the present study, a significant upregulation of miR-139-3p expression was demonstrated in the left ventricular myocardium of two-kidney one-clip (2K1C) hypertensive rats using microarray and quantitative real-time PCR (qRT-PCR). Based on computational analysis, we observed that miR-139-3p can control the expression of mitogen-activated protein kinase 1 (MAPK1) as a target gene, which is essential for the induction of cardiac hypertrophy and cardiomyocyte apoptosis. This study provides first information that the highly expressed miR-139-3p might be closely involved in MAPK1-mediated cardiac hypertrophy and cardiomyocyte apoptotic processes in 2K1C rat