Open AccessBRCA1 and TOP2A gene amplification and protein expression in four molecular subtypes of breast cancer
Author(s) -
Olivera Mitrović,
Mileva Mićić,
Vladan P. Čokić,
Vesna Koko,
Dragoslava Djikić,
Mirela Budeč,
Sanja Vignjević,
Tijana Breković
Publication year - 2013
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs1302511m
Subject(s) - immunohistochemistry , gene duplication , breast cancer , cancer research , gene , biology , microbiology and biotechnology , cancer , triple negative breast cancer , genetics , immunology
We studied TOP2A amplification (using FISH methods), and TOP2A and BRCA1 protein overexpression (immunohistochemistry) in four molecular subtypes of breast cancer. Of 53 patients, 32 showed TOP2A and 38 showed BRCA1 overexpression. The highest percentage of TOP2A amplification (47.8%) and deletion (13%) was detected in Luminal B subtypes. Of 11 Luminal B tumors with TOP2A amplification, 9 (81.8%) overexpressed TOP2A. BRCA1 protein overexpression showed significant positive correlation with TOP2A protein expression. BRCA1 and TOP2A proteins exhibited similar patterns of expression in Luminal B and triple-negative breast cancer, suggesting the same prognosis in those patients. [Projekat Ministarstva nauke Republike Srbije, br. 175053