Open AccessThe effect of different concentrations of Liberase HI used in a non automated method for human adult pancreatic islet isolation
Author(s) -
D. Nikolic,
Predrag Djordjevič,
Vesna Dimitrijević-Srećković,
Ivan Paunović,
Nevena Kalezić,
Srdjan Popović,
Djordje Stefanović,
Marina Dzingalasevic
Publication year - 2010
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs1003833n
Subject(s) - islet , biology , endocrinology , collagenase , medicine , andrology , insulin , biochemistry , enzyme
Liberase HI has many advantages over traditional collagenases. Liberase performs the rapid dissociation of human pancreata and allows for the preservation of the morphological and functional integrity of isolated islets. The aim of this study was to investigate the effect of different concentrations of Liberase HI (1.5, 2.5 and 3.5 mg/ml) on the yield, viability and function of islets isolated by a non-automated method. Based on our results, the Liberase HI concentration 1.5 mg/ml is recommended for this method of isolation.