Open AccessAnalysis of K-ras gene codon 12 mutation in pancreatic tissue of patients with pancreatic cancer
Author(s) -
Aleksandra Nikolić,
S Knĕzević,
Mila Krsmanovic,
Marjan Micev,
Momčilo Ristanović,
Dragica Radojković
Publication year - 2009
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs0904623n
Subject(s) - pancreatic cancer , mutation , restriction fragment length polymorphism , pancreas , polymerase chain reaction , ca19 9 , gene , biology , pancreatic disease , cancer , microbiology and biotechnology , cancer research , genetics , pathology , medicine , endocrinology
The aim of this study was to analyze K-ras codon 12 mutation in the pancreatic tissue of Serbian patients with pancreatic cancer and assess whether the given mutation can be used as a molecular marker for this disease. The study was performed on pancreatic tissue samples obtained from 40 patients with clinical diagnosis of pancreatic cancer. The presence of K-ras codon 12 mutation was analyzed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Our study showed that K-ras mutation is present with a high frequency (66%) in the pancreatic tissue of patients with pancretic cancer