Open AccessEffects of anoxia on 31P NMR spectra of Phycomyces blakesleeanus during development
Author(s) -
Marina Stanić,
Miroslav Živić,
Joana Zakrzewska
Publication year - 2009
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs0901017s
Subject(s) - phycomyces blakesleeanus , phycomyces , mycelium , chemistry , nuclear magnetic resonance spectroscopy , hydrolysis , biochemistry , biology , botany , stereochemistry , mutant , gene
The method of 31P NMR spectroscopy was used to investigate the effects of anoxia on Phycomyces blakesleeanus mycelium during development. The greatest changes were recorded in the PPc, NADH, and α-ATP signals. Decrease of PPc signal intensity is due to chain length reduction and reduction in number of PPn molecules. Smaller decrease of β-ATP compared to α-ATP signal intensity can be attributed to maintenance of ATP concentration at the expense of PPn hydrolysis. Sensitivity to anoxia varies with the growth stage. It is greatest in 32-h and 44-h mycelium, in which PPn is used as an additional energy source, while the smallest effect was noted for 36-h fungi