Open AccessTrans-activation of the human SOX3 promoter by MAZ in NT2/D1 cells
Author(s) -
Nataša KovačevićGrujičić,
Kazunari K. Yokoyama,
Milena Stevanović
Publication year - 2008
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs0803379k
Subject(s) - transcription factor , zinc finger , promoter , gene , biology , transcription (linguistics) , regulation of gene expression , regulator , microbiology and biotechnology , gene expression , genetics , linguistics , philosophy
In this study, we examine the role of three highly conserved putative binding sites for Myc-associated zinc finger protein (MAZ) in regulation of the human SOX3 gene expression. Electrophoretic mobility shift and supershift assays indicate that complexes formed at two out of three MAZ sites of the human SOX3 promoter involve ubiquitously expressed MAZ protein. Furthermore, in cotransfection experiments we demonstrate that MAZ acts as a positive regulator of SOX3 gene transcription in both undifferentiated and RA-differentiated NT2/D1 cells. Although MAZ increased both basal and RA-induced promoter activity, our results suggest that MAZ does not contribute to RA inducibility of the SOX3 promoter during neuronal differentiation of NT2/D1 cells