Open AccessDynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expression
Author(s) -
Svetlana Dinić,
Mirjana Mihailović,
Svetlana Ivanovič-Matić,
Aleksandra Uskoković,
Nevena Grdović,
Melita Vidaković,
Goran Poznanović
Publication year - 2008
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs0803355d
Subject(s) - nuclear matrix , transcription factor , biology , promoter , transcription (linguistics) , gene , gene expression , microbiology and biotechnology , general transcription factor , nuclear protein , tcf4 , genetics , linguistics , philosophy , chromatin
Participation of the nuclear matrix in regulation of alpha-2-macroglobulin (α2M) gene transcription during rat liver development and the acute-phase (AP) response are examined. DNA affinity chromatography of fetal and adult liver internal nuclear matrix proteins under basal and AP conditions with the α2M gene promoter (-852/+12) and immunoblot analysis revealed diverse patterns of association of transcription factors with the nuclear matrix. HNF-6, C/EBPα, and STAT5b were involved in basal and C/EBPβ, STAT1, and STAT3 in AP-stimulated α2M expression. These findings support the assumption that transcription factor-nuclear matrix interactions serve to channel gene regulatory proteins to DNA sequences