Open AccessTwo metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology
Author(s) -
Dragana B. Majić,
Jelena Samardžić,
Milan Milisavljević,
Aleksandar Krstić,
Vesna Maksimović
Publication year - 2008
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs0801077m
Subject(s) - metallothionein , complementary dna , biology , real time polymerase chain reaction , cdna library , gene , gene expression , gene family , transcriptome , microbiology and biotechnology , genetics , computational biology
Metallothioneins (MTs) are an extensive and diverse family of small cysteine-rich proteins with metal-binding ability that are involved in metal homeostasis and detoxification. Two cDNA clones of the MT3 type, differing in 3’ UTRs, were isolated from the developing buckwheat seed cDNA library. Following sequence analyses, expression profiles during flooding stress were monitored by Real Time RT PCR technology