Open AccessRat liver tyrosine aminotransferase activity and induction by dexamethasone upon cadmium intoxication
Author(s) -
S Jadranka Dundjerski,
Jelena Predic,
Aleksandra Čvoro,
Marko Matic
Publication year - 2003
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs0302003d
Subject(s) - tyrosine aminotransferase , dexamethasone , cytosol , cadmium , chemistry , endocrinology , medicine , enzyme inducer , in vitro , enzyme , basal (medicine) , biology , biochemistry , organic chemistry , insulin
This study was focused on Cd effects on basal and dexamethasone-induced tyrosine aminotransferase (TAT) activity in the rat liver cytosol. Cadmium (Cd), applied in the dose of 2 mg/kg b.w., stimulated both TAT activity and its induction by dexamethasone, inducing the most prominent alterations 24 h after administration. Doses lower than 2 mg Cd/kg b.w. were ineffective while the higher ones (3 and 4 mg Cd/kg b.w) led to the changes similar to those reached by 2 mg Cd/kg. The in vitro application of different Cd concentrations to the liver cytosol rendered the enzyme activity unchanged suggesting that the metal acted at the level of TAT gene transcription