Open AccessPlant growth-promoting activity in bean plants of endophytic bacteria isolated from Echeveria laui
Author(s) -
Anderson Emmer,
João Arthur dos Santos de Oliveira,
Andressa Domingos Polli,
Julio Cesar Polônio,
Leonardo Hamamura Alves,
Cintia Zani Fávaro Polonio,
João Lúcio de Azevedo,
João Alencar Pamphile
Publication year - 2021
Publication title -
acta brasiliensis
Language(s) - English
Resource type - Journals
eISSN - 2526-4338
pISSN - 2526-432X
DOI - 10.22571/2526-4338496
Subject(s) - biology , botany , phaseolus , shoot , bacteria , 16s ribosomal rna , horticulture , genetics
Echeveria laui (Crassulaceae) is commonly commercialized due to its drought-tolerance capacity and to its rosette-shaped aesthetics. Since endophytes associated with plants from a dry or arid environment have scarcely been analyzed as yet, current research comprises the isolation of leaf endophytic bacteria from E. laui (one five-year-old and one two-year-old plants) investigating plant growth-promoting endophytic bacteria which may solubilize phosphate, fix nitrogen, produce exopolysaccharides/IAA and antagonize phytopathogens. Isolation by the maceration methodology provided a colonization rate of 1.98 x109 CFU g-1 for the two-year-old plant and 1.14 x 1010 CFU g-1 for the five-year-old one. All 40 isolates evaluated showed in vitro plant growth-promoting agent’s abilities, with emphasis on EG04, ELG18, and ELP06. The capacity of the three best bacterial isolates were evaluated under greenhouse conditions in common and black bean (Phaseolus vulgaris L.) plants. Based on the sequencing of the 16S rRNA region and phylogenetic analysis, the three endophytes were identified as Pantoea sp. (ELG04 and ELG18) and Erwinia sp. (ELP06). Under greenhouse conditions, statistically significant differences were found among the plants treated with the three endophytes when compared to control plants for fresh and dry shoot, root biomass and length.