Open AccessSTUDI PERBANDINGAN ANALISIS VITAMIN E MINYAK SAWIT MERAH TERSAPONIFIKASI ANTARA METODE SPEKTROFOTOMETRI UV-VIS DAN KCKT
Author(s) -
Arlina Mayharty Andulaai,
Ruslan Ruslan,
Hardi Ys,
Dwi Juli Puspitasari
Publication year - 2017
Publication title -
kovalen
Language(s) - English
Resource type - Journals
ISSN - 2477-5398
DOI - 10.22487/j24775398.2017.v3.i1.8233
Subject(s) - saponification , chromatography , chemistry , high performance liquid chromatography , spectrophotometry , hexane , solvent , organic chemistry
A research about a comparative study of spectrophotometry UV-Vis and HPLC method for the analysis of vitamin E in saponified red palm oil has been done. This research aims to compare the results of analysis using Spectrophotometer UV - Vis and HPLC to determine the concentration of vitamin E in red palm oil previously saponified and extracted. HPLC analysis was carried out using an RP-18 column and mobile phase composed a methanol and water ( 86:14 ), with a flow rate of 1 ml/min and UV detection at 290 nm. For the Spectrophotometric UV-Vis analysis, hexane was used as a solvent and the wavelength at 298,5 nm was selected for the detection. The results are the concentration of vitamin E using spectrophotometric and HPLC method was respectively 104.5 ppm and 127 ppm.Keyword: Vitamin E, Red Palm Oil, saponification, extraction, spectrophotometry Ultra Violet -Visible, High Performance Liquid Chromatography