Open AccessGREEN ANALYTICAL METHOD FOR QUANTIFICATION OF SECNIDAZOLE IN TABLETS BY HPLC-UV
Author(s) -
Jéssica de Carvalho Lima,
Ana Carolina Kogawa,
Hérida Regiunes Salgado
Publication year - 2018
Publication title -
drug analytical research
Language(s) - English
Resource type - Journals
ISSN - 2527-2616
DOI - 10.22456/2527-2616.89411
Subject(s) - chromatography , chemistry , high performance liquid chromatography , acetic acid , solvent , particle size , biochemistry , organic chemistry
A simple, rapid, economic and green analytical method was validated for the determination of secnidazole in tablets. The aim was to contribute to the green analytical chemistry since it has low use of organic solvent and low production of toxic waste. For the HPLC-UV method, the mobile phase was a mixture of purified water + 0.7 % acetic acid and ethanol (78:22, v/v), ow rate was 1.3 mL min-1 on column CN Phenomenex Luna (250 x 4.60 mm, 5 μm particle size), injection volume was 20 μL with UV detection at 318 nm and retention time of 4.26 minutes. The method was linear over the concentration range of 5-100 μg mL-1 (r = 0.9998) with limits of detection and quantitation of 0.533 e 1.615 μg mL-1, respectively. The precision of the method showed RSD less than 2 %. The accuracy determined by the average recoveries was 99.58 %. The secnidazole tablets were subjected to oxidation, acid, alkaline, neutral and photolysis degradation as stress conditions and the method was considered as indicative of stability. The method is adequate and safe to be a great alternative method in routine quality control analyzes for determination and quantification of secnidazole tablets.