Open AccessAnalysis of seized stanozolol formulations in South Brazil by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry
Author(s) -
Everton Rafael Campos,
Sarah Eller,
Letícia Birk,
M. A. Coimbra,
Sandra Manoela Dias Macedo,
Maurı́cio Yonamine,
Tiago Franco de Oliveira
Publication year - 2020
Publication title -
drug analytical research
Language(s) - English
Resource type - Journals
ISSN - 2527-2616
DOI - 10.22456/2527-2616.108853
Subject(s) - stanozolol , chromatography , anabolic androgenic steroids , chemistry , liquid chromatography–mass spectrometry , anabolic steroid , quadrupole time of flight , mass spectrometry , tandem mass spectrometry , anabolism , biochemistry
Anabolic-androgenic steroids (AAS) are synthetic derivatives of testosterone which are used medically for several diseases. However, misuse is commonly observed by athletes to promote enhancement of strength and performance. AAS are frequently obtained through online black markets from clandestine drug manufacturing laboratories, without any quality standards, being potentially dangerous for users. The purpose of this work was the development and application of a fast and simple procedure for the quantitation of stanozolol by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry (LC-QTOF-MS) in tablets packs seized in Rio Grande do Sul state, Brazil. The samples of stanozolol were separated considering its dosage form. The internal standard (methyltestosterone) was added to the aliquots of the samples, dissolved in methanol and 5μL were injected into the analytical system. The newly developed method has been validated for lower limit of quantitation (LLOQ), linearity, accuracy, precision and selectivity. The LLOQ was 0.1 µg/mL. The developed method was successfully applied to 31 samples seized by the Secretaria da Receita Federal do Brasil (a Brazilian federal revenue service agency). According to the results, 90.3% of the suspected medicines (n=31) were adulterated, and 65% exhibited higher concentrations of stanozolol than those indicated in the label. This work successfully established a new method for quantification of stanozolol using LC-QTOF-MS. This method aims at contributing to the identification and quantification of this anabolic androgenic steroid frequently seized by federal inspection agencies.