Open AccessGenotoxicity effects of medicinal plants extracts against bacterial species, Mycoplasma hominis
Author(s) -
Mohamed Yassin,
Youssef O. AlGhamdi,
Essam H. Mohamed,
Seyed Mostafa,
A. Merghani,
H Amer,
Saad H. Alotaibi,
Mohamed Mohamed Soliman,
H. Nasr-eldeen,
Maqbool Hassan
Publication year - 2021
Publication title -
journal of environmental biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.247
H-Index - 48
eISSN - 2394-0379
pISSN - 0254-8704
DOI - 10.22438/jeb/42/2/mrn-1663
Subject(s) - mycoplasma hominis , antimicrobial , genotoxicity , traditional medicine , biology , mycoplasma , microbiology and biotechnology , medicinal plants , botany , chemistry , toxicity , medicine , organic chemistry
Aim: To assess the antimicrobial activity and genotoxicity of three medicinal plants used by Saudi Arabian people as traditional medicine against Mycoplasma hominis. Methodology: Different concentrations of Syzygium aromaticum (clove), Vachellia nilotica (acacia), and Thyme vulgaris (thyme) extracts were used as antimicrobial agents against M. hominis, and their lethal effects on Mycoplasma genome DNA were analyzed using repetitive element PCR(Rep-PCR). Results: The aqueous extracts of clove and Acacia at 3.125 mg ml-1 were found to be active antimicrobials against three tested Mycoplasm. Thyme extract exhibited antimicrobial activity at 12.5 mg ml-1. Moreover, this extract revealed potent lethal activities as growth turbidity decreased with increasing concentration or exposure time as compared to untreated Mycoplasma. The results of Rep-PCR clearly indicate that changes occured in the number of genetic bands in treated Mycoplasma at certain concentrations as compared to untreated Mycoplasma.Interpretation: These results indicate the possibility of using these extracts as a source of antibacterial compounds for treating infections caused by Mycoplasma.Key words: Antimicrobial activity, Genotoxicity, Mycoplasma hominis, Medicinal plants, S. aromaticum