Open AccessDevelopment of species-specific marker and molecular diversity of Conogethes punctiferalis and Conogethes sahyadriensis (Lepidoptera: Crambidae)
Author(s) -
Vasudev Kammar,
Dayananda Sagar,
Rahul Kumar Chandel,
P. R. Shashank,
A. K. Chakravarthy
Publication year - 2021
Publication title -
journal of environmental biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.247
H-Index - 48
eISSN - 2394-0379
pISSN - 0254-8704
DOI - 10.22438/jeb/42/2/mrn-1322
Subject(s) - biology , genetic diversity , molecular marker , phylogenetic tree , evolutionary biology , genetic marker , genetics , computational biology , gene , population , demography , sociology
Aim: The aim of this study was to develop species specific marker for quick identification of two Conogethes species and to study their molecular diversity. Methodology: Species-specific markers were developed using mitochondrial cytochrome oxidase 1 (COI) partial gene sequences of Conogethes punctiferalis and C. sahyadriensis and they were validated. Phylogenetic tree was constructed using MEGA X program, tree robustness was evaluated by bootstrapping with 1000 replicates with all sequences of C. punctiferalis and C. sahyadriensis.Results: Bioinformatic analysis of C. punctiferalis and C. sahyadriensis COI sequences revealed that they have sufficient genetic variability to develop species specific marker. The COI based species-specific markers amplified an expected fragment size of 333 bp for C. punctiferalis and 522 bp for C. sahyadriensis, which clearly differentiate these two species.Interpretation: The species specific marker developed in this study will be useful in quick identification of C. punctiferalis and C. sahyadriensis. The use of COI gene as a marker can provide a better estimate of genetic diversity, as it is maternally inherited and can be helpful to understand evolutionary processes.Key words: Conogethes punctiferalis, C. sahyadriensis, MtCOI, Molecular diversity, Species-specific marker